Background: This study aimed to investigate the effectiveness of the prevention and relationship enhancement program (PREP) on marital burnout in married women. Methods: Thirty married women were selected and randomly replaced in experimental (n=15) and control (n=15) groups. The quasiexperimental research method was conducted in the pretest-posttest control group design to examine the effectiveness of the PREP on marital burnout in married women. The sessions of the PREP were implemented for the experimental group during 8 sessions of 90 minutes, one session per week, and no educational intervention was performed for the control group. Multivariate analysis of covariance was used as a statistical method. Results: The results showed that there was a significant difference between the control and experimental groups in psychological and emotional aspects of marital burnout(Pvalue≤, 0. 02). Our results also showed that the total burnout score was significantly different from pre-test to post-test in the experimental group (Pvalue≤, 0. 01). Conclusions: It can be implied from the results of our study that PREP can have different implications in Iranian culture.